3QQ5

Crystal structure of the [FeFe]-hydrogenase maturation protein HydF

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793, 0.9795, 0.9769
Spacegroup name	P 2 3
Unit cell lengths	138.259, 138.259, 138.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.710 - 2.990
R-factor	0.274
Rwork	0.274
R-free	0.30900
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.700
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.820	3.150
High resolution limit [Å]	2.990	2.990
Rmerge	0.120	0.484
Number of reflections	18028
<I/σ(I)>	20.9	3.6
Completeness [%]	99.9	100
Redundancy	31.9	32.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.8	293	8% PGA-LM (Poly-L-glutamic acid, low molecular weight range), 0.2 M Na-Formate, 0.1 M Tris buffer, pH 7.8, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	7.8	293	8% PGA-LM (Poly-L-glutamic acid, low molecular weight range), 0.2 M Na-Formate, 0.1 M Tris buffer, pH 7.8, VAPOR DIFFUSION, temperature 293K