3QOD
Crystal Structure of Heterocyst Differentiation Protein, HetR from Fischerella mv11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 65 |
Unit cell lengths | 123.383, 123.383, 109.615 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.387 - 3.380 |
R-factor | 0.207 |
Rwork | 0.204 |
R-free | 0.26700 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.896 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.410 |
High resolution limit [Å] | 3.350 | 3.350 |
Number of reflections | 13425 | |
<I/σ(I)> | 9.3 | 3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.7 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 289 | 1.0 M ammonium sulphate, 0.1 M imidazole pH 8.0, 0.2 M sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 289K |