3QNU

Crystal structure of the cytosolic domain of human atlastin-1 in complex with GDP, hexagonal form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-14
Detector	MAR CCD 165 mm
Wavelength(s)	0.9793, 0.9795
Spacegroup name	P 65 2 2
Unit cell lengths	144.889, 144.889, 103.694
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.966 - 2.800
R-factor	0.2385
Rwork	0.235
R-free	0.30610
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.466
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	50.000	3.020
High resolution limit [Å]	2.800	4.790	2.800
Rmerge	0.071	0.040	0.330
Number of reflections	16292
<I/σ(I)>	14.5	122	6.4
Completeness [%]	99.9	100	99.9
Redundancy	13.6	15	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1M Tris-HCl, pH8.5, 25%(w/v) PEG1500, 20%(v/v) Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K