3QNF
Crystal structure of the open state of human endoplasmic reticulum aminopeptidase 1 ERAP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 97.246, 132.816, 233.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 3.000 |
| R-factor | 0.23133 |
| Rwork | 0.229 |
| R-free | 0.28264 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2XDT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.087 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.153 | 0.668 |
| Number of reflections | 60032 | |
| <I/σ(I)> | 5.8 | 1.6 |
| Completeness [%] | 97.8 | 98.8 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1M HEPES pH 7.5; 25% PEG 3350, VAPOR DIFFUSION, temperature 293K |
