3QNE

Candida albicans seryl-tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-07
Detector	ADSC QUANTUM 210
Spacegroup name	P 61 2 2
Unit cell lengths	90.050, 90.050, 276.757
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.939 - 2.000
R-factor	0.1673
Rwork	0.165
R-free	0.20650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dq0
RMSD bond length	0.010
RMSD bond angle	1.157
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.100	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.069	0.230
Number of reflections	43773
<I/σ(I)>	7.8	3.1
Completeness [%]	95.7	78
Redundancy	21.8	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	298	3.2-3.4 M ammonium sulfate, 0-2% v/v glycerol, 100 mM MES/sodium, pH 5.6-5.8, VAPOR DIFFUSION, temperature 298K