3QN9
Crystal structure of a 6-pyruvoyltetrahydropterin synthase homologue from Esherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-13 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.123 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 112.589, 112.589, 68.818 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.752 - 2.930 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.26503 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oba |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.029 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.500 | 48.752 |
| High resolution limit [Å] | 2.924 | 2.294 |
| Rmerge | 0.231 | |
| Number of reflections | 10571 | |
| Completeness [%] | 94.8 | 94.8 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.02M MgCl2, 0.1M HEPES pH7.5, Polyacryslic acid 5100 sodium salt, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
