3QMX
X-ray crystal structure of Synechocystis sp. PCC 6803 Glutaredoxin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2010-06-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.278, 39.124, 50.827 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.060 - 1.822 |
R-factor | 0.1664 |
Rwork | 0.163 |
R-free | 0.19560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3grx |
RMSD bond length | 0.005 |
RMSD bond angle | 0.952 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.060 |
High resolution limit [Å] | 1.822 |
Rmerge | 0.077 |
Number of reflections | 6614 |
Completeness [%] | 94.1 |
Redundancy | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1% PEG 4000, 0.1M HEPES, 1.25M Ammonium Sulfate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |