Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0072, 1.0090 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 233.791, 233.791, 233.791 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| Rwork | 0.194 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.480 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 18820 | |
| <I/σ(I)> | 51.1 | 14.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 45.5 | 44.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | reservoir solution contains 12.5% (v/v) PEG4000, 100 mM Hepes. Protein (10 mg/ml) in 20 mM Hepes, 150 mM NaCl, and 5 mM BME, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
