3QLU
Crystal structure of the GluK2/GluK5 (GluR6/KA2) ATD dimer assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.626, 139.548, 195.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.970 - 2.906 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3OM0 and 3H6H |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.918 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.114 | 0.450 |
| Number of reflections | 40232 | |
| <I/σ(I)> | 11.6 | 2.9 |
| Completeness [%] | 99.1 | 97.1 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 0.15 M Ammonium Sulfate; 0.1 M Tris; 18% PEG 4K, pH 8.20, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
