3QLT

Crystal structure of a GluK2 (GluR6) glycan wedge homodimer assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03320
Spacegroup name	I 4
Unit cell lengths	191.805, 191.805, 47.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.951 - 2.988
R-factor	0.2441
Rwork	0.242
R-free	0.29320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h6g
RMSD bond length	0.003
RMSD bond angle	0.608
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	2.988	2.990
Rmerge	0.078	0.698
Number of reflections	17783
<I/σ(I)>	16	2
Completeness [%]	99.9	99.4
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10% PEG 8K, 20% ethylene glycol, 0.02 M each 1,6-hexanediol, 1-butanol, (RS)-1,2-propanediol, 2-propanol, 1,4 butanediol, 1,3-propanediol, and 0.1 M MOPS/HEPES-Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K