3QLK

Crystal Structure of RipA from Yersinia pestis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	200
Detector technology	CCD
Collection date	2009-11-14
Detector	ADSC QUANTUM 315
Spacegroup name	P 31 2 1
Unit cell lengths	197.508, 197.508, 61.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.716 - 3.000
R-factor	0.1864
Rwork	0.184
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.627
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.120	0.040	0.563
Number of reflections	27624
<I/σ(I)>	7.2
Completeness [%]	100.0	100	100
Redundancy	14.6	14.6	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	298	18% PEG 3350, .2M Na Citrate, .1M BisTris Propane pH 6.3 and 1mM CoA, VAPOR DIFFUSION, HANGING DROP, temperature 298K