3QLE
Structural Basis for the Function of Tim50 in the Mitochondrial Presequence Translocase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-19 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 84.109, 84.109, 116.549 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.989 - 1.831 |
| R-factor | 0.1762 |
| Rwork | 0.175 |
| R-free | 0.20160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ghq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.186 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_351)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.830 |
| Number of reflections | 21756 |
| Completeness [%] | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 290 | 100 mM MES buffer (pH 6.0), 30% (w/v) PEG4K and 0.2 M ammonium acetate, EVAPORATION, temperature 290K |
