3QKX

Crystal structure of a TetR-family transcriptional regulator (HI0893) from Haemophilus influenzae RD at 2.35 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97954,0.91837,0.97917
Spacegroup name	P 62
Unit cell lengths	71.160, 71.160, 159.334
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.560 - 2.350
R-factor	0.224
Rwork	0.223
R-free	0.24840
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.205
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.560	29.560	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge		0.013	0.013
Total number of observations		1539	10667
Number of reflections	19028
<I/σ(I)>	13	36.3	1.6
Completeness [%]	99.9	95.2	99.9
Redundancy	7.7	7.2	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.33	277	1.80 M lithium sulfate, 0.1M HEPES pH 7.33, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K