3QKU
Mre11 Rad50 binding domain in complex with Rad50 and AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 177.398, 177.398, 130.423 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| R-factor | 0.26052 |
| Rwork | 0.258 |
| R-free | 0.30838 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1us8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.223 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 153.600 | 3.387 |
| High resolution limit [Å] | 3.300 | 3.301 |
| Rmerge | 0.048 | 0.660 |
| Number of reflections | 18667 | |
| <I/σ(I)> | 35.7 | 3.6 |
| Completeness [%] | 99.7 | 99.41 |
| Redundancy | 9.8 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris, 200-300 mM lithium sulfate, 12-13% PEG3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
