3QJX

Crystal Structure of E. coli Aminopeptidase N in complex with L-Serine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-05
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 31 2 1
Unit cell lengths	120.230, 120.230, 170.801
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.740 - 1.450
R-factor	0.12108
Rwork	0.120
R-free	0.14709
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.032
RMSD bond angle	2.601
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.740	35.740	1.530
High resolution limit [Å]	1.450	4.590	1.450
Rmerge	0.062	0.025	0.537
Number of reflections	244035
<I/σ(I)>	13.9	36.4	2.8
Completeness [%]	99.9	99.8	99.8
Redundancy	5.4	5.4	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1.8M Sodium Malonate, L-serine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K