3QJL

One RAMP protein binding different RNA substrates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.5621
Spacegroup name	P 43 21 2
Unit cell lengths	88.773, 88.773, 257.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.700 - 2.701
R-factor	0.2216
Rwork	0.218
R-free	0.26410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.152
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: dev_589))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.700	2.700
Number of reflections	28452
Completeness [%]	89.0	89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	300	0.005 M MgSO4, 0.05 M MES (pH6.0), 3-8% PEG4000, 0.2 M NaCl and 0.1 M KCl, VAPOR DIFFUSION, HANGING DROP, temperature 300K