3QIM
Histidine 416 of the periplamsic binding protein NikA is essential for nickel uptake in Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-23 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.731, 94.005, 124.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.840 - 2.100 |
| R-factor | 0.17911 |
| Rwork | 0.176 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zlq |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.541 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 24.840 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 56622 |
| <I/σ(I)> | 12.93 |
| Completeness [%] | 93.0 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 1.6M Ammonium sulfate, 100 mM sodium acetate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
