3QIB

Crystal structure of the 2B4 TCR in complex with MCC/I-Ek

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-11
Detector	ADSC QUANTUM 315r
Spacegroup name	F 2 2 2
Unit cell lengths	146.749, 167.178, 259.489
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.970 - 2.700
R-factor	0.222
Rwork	0.221
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.304
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	43820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.2	295	50% PEG200, 0.2 M sodium chloride, 0.1 M sodium potassium phosphate, pH 6.2, VAPOR DIFFUSION, temperature 295K