3QHS

Crystal structure of full-length Hfq from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.933
Spacegroup name	P 1
Unit cell lengths	61.910, 62.150, 81.260
Unit cell angles	78.56, 86.16, 59.86

Refinement procedure

Resolution	45.214 - 2.850
R-factor	0.20919
Rwork	0.207
R-free	0.24969
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hk9
RMSD bond length	0.010
RMSD bond angle	1.055
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	2.920
High resolution limit [Å]	2.850	2.850
Rmerge	0.128	0.320
Number of reflections	23973
<I/σ(I)>	14.22	5.6
Completeness [%]	92.9	83.7
Redundancy	7.03	6.43

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	30% v/v PEG 550 MME, 0.05M calcium chloride dihydrate, 0.1M BIS-TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K