3QHE

Crystal structure of the complex between the armadillo repeat domain of adenomatous polyposis coli and the tyrosine-rich domain of Sam68

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-03-30
Detector	PSI PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 61
Unit cell lengths	148.223, 148.223, 63.379
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.180 - 2.400
R-factor	0.18915
Rwork	0.187
R-free	0.23046
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3au3
RMSD bond length	0.010
RMSD bond angle	1.091
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.180	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.095	0.388
Number of reflections	31344
<I/σ(I)>	7.1	2
Completeness [%]	100.0	100
Redundancy	11.2	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	100mM BIS-TRIS (pH 5.5), 150mM NaCl, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K