3QGJ
1.3A Structure of alpha-Lytic Protease Bound to Ac-AlaAlaPro-Alanal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-28 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 63.936, 63.936, 316.897 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.100 - 1.300 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h5c |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.960 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.100 |
| High resolution limit [Å] | 1.300 |
| Number of reflections | 85626 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.8 | 277 | 1.2M Li2SO4, 20mM Tris-SO4, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
