3QFU
Crystal structure of Yeast Hsp70 (Bip/kar2) complexed with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-04-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.367, 85.667, 94.921 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.490 - 1.800 |
| R-factor | 0.21765 |
| Rwork | 0.217 |
| R-free | 0.23771 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ldn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.957 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.457 | |
| Number of reflections | 46029 | |
| <I/σ(I)> | 21.8 | 3.3 |
| Completeness [%] | 94.6 | 88.1 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 0.1 M HEPES, 0.2 M Magnesium Chloride, 22% (w/v) PEG 6000 , pH 7, VAPOR DIFFUSION, HANGING DROP |
