3QFS

Crystal Structure of NADPH-Cytochrome P450 Reductase (FAD/NADPH domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-12
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	54.640, 58.540, 130.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.710 - 1.400
R-factor	0.211
Rwork	0.211
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1amo
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	73930
<I/σ(I)>	32.5	2.6
Completeness [%]	89.6	82.8
Redundancy	9.2	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	292	100 mM magnesium chloride, 15% PEG5000 MME, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 292K