3QFA
Crystal structure of the human thioredoxin reductase-thioredoxin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0068 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.250, 105.030, 120.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.910 - 2.200 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2j3n and 1aiu |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 70734 |
<I/σ(I)> | 6.73 |
Completeness [%] | 83.8 |
Redundancy | 3.16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297.15 | 15% PEG 6000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 297.15 K |