3QEA

Crystal structure of human exonuclease 1 Exo1 (WT) in complex with DNA (complex II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.200
Spacegroup name	P 43 21 2
Unit cell lengths	71.810, 71.810, 181.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.905 - 3.100
R-factor	0.2139
Rwork	0.212
R-free	0.24350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.355
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	3.100	3.100
Number of reflections	16451
Completeness [%]	92.9	58.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	290	MES, PEG 6K, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MES
2	1	1	PEG 6K
3	1	2	MES
4	1	2	PEG 6K