3QCB
Human receptor protein tyrosine phosphatase gamma, domain 1, apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-15 |
| Detector | RIGAKU SATURN 92 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.900, 79.500, 123.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.660 - 2.100 |
| R-factor | 0.255 |
| Rwork | 0.254 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1RPM WITH LOOPS EXCISED AND NON- IDENTICAL SIDE CHAINS SHORN. |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.800 |
| Data reduction software | HKL-2000 ((DENZO)) |
| Data scaling software | HKL-2000 ((SCALEPACK)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.104 | 0.463 |
| Number of reflections | 43827 | |
| <I/σ(I)> | 18.8 | 3.2 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 6.4 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 277 | pH 6.5, vapor diffusion, temperature 277K |
