3QC7
The structure of bacteriophage phi29 head fibers has a supercoiled triple repeating helix-turn-helix motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.968 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 47.248, 47.248, 424.752 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.470 - 1.520 |
| R-factor | 0.19542 |
| Rwork | 0.194 |
| R-free | 0.21833 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.368 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.070 | 0.407 |
| Number of reflections | 28649 | |
| <I/σ(I)> | 33.4 | 4.6 |
| Completeness [%] | 98.6 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | The gp8.5 fragment crystals were grown in 100mM sodium phosphate at pH ~6.8 and 8% w/v PEG 3000. It took about one week for crystals to appear, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
