3QBZ
Crystal structure of the Rad53-recognition domain of Saccharomyces cerevisiae Dbf4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 83.740, 83.740, 103.669 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.823 - 2.692 |
| R-factor | 0.2264 |
| Rwork | 0.224 |
| R-free | 0.27290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oq4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.034 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.057 | 0.419 |
| Number of reflections | 6371 | |
| <I/σ(I)> | 31 | 3.5 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6.9 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.9 M ammonium sulfate 50 mM sodium cacodylate 15 mM CYMAL-7, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
