3QAW
Crystal structure of a glutathione-S-transferase from Antarctic clam Laternula elliptica in a complex with glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-06-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.393, 57.611, 55.449 |
| Unit cell angles | 90.00, 123.84, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| Rwork | 0.181 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qav |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.860 |
| Number of reflections | 11476 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 7% Tacsimate pH 8.0, 24% (w/v) polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
