3QAM
Crystal Structure of Glu208Ala mutant of catalytic subunit of cAMP-dependent protein kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.412, 79.639, 60.800 |
| Unit cell angles | 90.00, 113.28, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.920 |
| Rwork | 0.195 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1atp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.051 | 0.440 |
| Number of reflections | 32026 | |
| <I/σ(I)> | 17 | 2.2 |
| Completeness [%] | 88.8 | 65.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 10-12% polyethylene glycol 6000, 100 mM Tris-HCl, 10 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
