3Q9U
In silico and in vitro co-evolution of a high affinity complementary protein-protein interface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Detector technology | CCD |
| Collection date | 2009-06-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8729 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.901, 57.620, 58.230 |
| Unit cell angles | 89.96, 90.14, 113.44 |
Refinement procedure
| Resolution | 37.738 - 2.300 |
| R-factor | 0.2441 |
| Rwork | 0.240 |
| R-free | 0.31750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q9n |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.327 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 27228 |
| Completeness [%] | 83.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.2 M NaF 20 % PEG 3350, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
