3Q98
Structure of ygeW encoded protein from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-13 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 63 |
| Unit cell lengths | 78.250, 78.250, 100.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.067 - 2.001 |
| R-factor | 0.1791 |
| Rwork | 0.175 |
| R-free | 0.22550 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.056 |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.091 | 0.408 |
| Number of reflections | 23411 | |
| <I/σ(I)> | 15.5 | 5.8 |
| Completeness [%] | 99.5 | 96.1 |
| Redundancy | 9.1 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 20% PEG3350, 200 mM MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
