3Q93
Crystal Structure of Human 8-oxo-dGTPase (MTH1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-16 |
| Detector | RAYONIX MX-225 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.544, 67.681, 82.678 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.960 - 1.800 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iry |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.066 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.960 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.108 | 0.701 |
| Number of reflections | 31366 | |
| <I/σ(I)> | 4.4 | 2.3 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 7.7 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 277 | 0.26 M AMMONIUM SULFATE, 22 % w/v PEG4000, 13 % GLYCEROL, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
