3Q88
Glucose-6-phosphate isomerase from Francisella tularensis complexed with ribose 1,5-bisphosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 114.465, 114.465, 84.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.990 - 1.700 |
| R-factor | 0.1476 |
| Rwork | 0.146 |
| R-free | 0.17390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ljk |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.695 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.108 | 0.822 |
| Number of reflections | 70571 | |
| <I/σ(I)> | 10.1 | 2.69 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.2 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 0.2 M Ca(OAc)2, 0.1 M MES buffer, 10% 2-propanol, 0.01 M 5-phospho-alpha-D-ribose 1-diphosphate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
