3Q7K
Formate Channel FocA from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-08-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.951, 205.723, 106.046 |
| Unit cell angles | 90.00, 115.49, 90.00 |
Refinement procedure
| Resolution | 43.640 - 2.800 |
| R-factor | 0.22034 |
| Rwork | 0.219 |
| R-free | 0.25366 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kcu |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.837 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.800 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.089 |
| Number of reflections | 92780 |
| <I/σ(I)> | 7.8 |
| Completeness [%] | 92.3 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | PEG 400, lithium sulfate, sodium citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
