3Q78

Cryptococcus neoformans protein farnesyltransferase in complex with FSPP and DDPTASACNIQ peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9710
Spacegroup name	P 43 21 2
Unit cell lengths	143.020, 143.020, 130.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.227 - 2.200
R-factor	0.1843
Rwork	0.183
R-free	0.21420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.656
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.6.1_336)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	65570
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	290	15% PEG4K, 100mM CAPSO pH 9.5, 100 mm NaCl, 150 mM Lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 290K