3Q75
Cryptococcus neoformans protein farnesyltransferase in complex with FPT-II and TKCVVM peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 141.915, 141.915, 130.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.450 - 2.140 |
| R-factor | 0.214 |
| Rwork | 0.213 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 70375 | |
| Completeness [%] | 95.8 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 290 | 15% PEG4K, 100mM CAPSO pH 9.5, 100mM NaCl, 150mM Lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
