3Q6N
Crystal Structure of Human MC-HSP90 in P21 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-24 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 157.897, 90.868, 167.093 |
Unit cell angles | 90.00, 115.85, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.050 |
R-factor | 0.20721 |
Rwork | 0.204 |
R-free | 0.25921 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q6m |
RMSD bond length | 0.012 |
RMSD bond angle | 1.398 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.160 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.058 | 0.362 |
Number of reflections | 82281 | |
<I/σ(I)> | 19.6 | 5.1 |
Completeness [%] | 98.8 | 99.4 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1.0M ammonium sulfate, 0.04M Tris-sodium citrate, 0.04 mM cisplatin, pH 5.4-5.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |