3Q6L

Crystal Structure of Human Adipocyte Fatty Acid Binding Protein (FABP4) at 1.4 Ang. Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	73
Detector technology	CCD
Collection date	2010-11-24
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9774
Spacegroup name	P 21 21 21
Unit cell lengths	32.647, 54.004, 75.237
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.870 - 1.400
R-factor	0.19158
Rwork	0.190
R-free	0.22728
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hk1
RMSD bond length	0.009
RMSD bond angle	1.251
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	54.100	54.100	1.480
High resolution limit [Å]	1.400	4.430	1.400
Rmerge	0.084	0.033	0.750
Number of reflections	25377
<I/σ(I)>	14.3	0.033	2.1
Completeness [%]	94.5	99.8	94.5
Redundancy	7.5	8.2	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	298	Sodium phosphate monobasic monohydrate/Potassium phosphate dibasic 2.2 - 2.6M, pH 7 - 7.4, Protein concentration 17mg/ml., VAPOR DIFFUSION, SITTING DROP, temperature 298K