3Q6K

Salivary protein from Lutzomyia longipalpis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-29
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 62 2 2
Unit cell lengths	120.012, 120.012, 244.747
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.520
R-factor	0.22039
Rwork	0.218
R-free	0.26787
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.011
RMSD bond angle	1.405
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.560
High resolution limit [Å]	2.520	6.830	2.520
Rmerge	0.081	0.044	0.679
Number of reflections	35867
<I/σ(I)>	11.4
Completeness [%]	99.3	99.7	88.6
Redundancy	23	23.3	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	298	10 % PEG 6000, 0.1 M sodium citrate, 6 mM Nickel chloride, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K