3Q6F
Crystal structure of Fab of human mAb 2909 specific for quaternary neutralizing epitope of HIV-1 gp120
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-02-18 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.03321 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 178.504, 178.504, 218.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.653 - 3.192 |
R-factor | 0.2013 |
Rwork | 0.199 |
R-free | 0.24290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.234 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 66903 | |
Completeness [%] | 99.5 | 78.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.8 | 296 | 14% PEG 8000, 0.1M HEPES, pH6.8, 0.1M glycine, and 8% ethylene glycol, under the oil, temperature 296K, EVAPORATION |
1 | EVAPORATION | 6.8 | 296 | 14% PEG 8000, 0.1M HEPES, pH6.8, 0.1M glycine, and 8% ethylene glycol, under the oil, temperature 296K, EVAPORATION |
1 | EVAPORATION | 6.8 | 296 | 14% PEG 8000, 0.1M HEPES, pH6.8, 0.1M glycine, and 8% ethylene glycol, under the oil, temperature 296K, EVAPORATION |
1 | EVAPORATION | 6.8 | 296 | 14% PEG 8000, 0.1M HEPES, pH6.8, 0.1M glycine, and 8% ethylene glycol, under the oil, temperature 296K, EVAPORATION |