3Q5Z
Crystal structure of virulent allele ROP5B pseudokinase domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-06 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 117.340, 70.553, 43.014 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.727 - 1.900 |
R-factor | 0.2123 |
Rwork | 0.209 |
R-free | 0.26130 |
Structure solution method | SIRAS |
RMSD bond length | 0.019 |
RMSD bond angle | 1.585 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.054 | 0.032 | 0.486 |
Number of reflections | 28779 | ||
<I/σ(I)> | 15.4 | ||
Completeness [%] | 98.7 | 99.6 | 88.7 |
Redundancy | 13.1 | 13.8 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 20% PEG-3350, 0.3M Sodium malonate, 0.01M MgCl2, pH 5.0, vapor diffusion, hanging drop, temperature 291K |