3Q54

Crystal structure of Escherichia coli BamB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-30
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9794
Spacegroup name	I 2 2 2
Unit cell lengths	49.150, 121.123, 133.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.196 - 2.004
R-factor	0.1754
Rwork	0.172
R-free	0.23160
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.071
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.000	2.070
High resolution limit [Å]	2.000	2.000
<I/σ(I)>	13.3
Completeness [%]	78.2	60.2
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2M Lithium sulfate monohydrate 0.1M BIS-TRIS, pH 6.5, 25%PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K