3Q49

Crystal structure of the TPR domain of CHIP complexed with Hsp70-C peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97924
Spacegroup name	P 21 21 2
Unit cell lengths	46.033, 77.967, 37.341
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.678 - 1.543
R-factor	0.1695
Rwork	0.168
R-free	0.20650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c2l
RMSD bond length	0.005
RMSD bond angle	0.865
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.700	1.570
High resolution limit [Å]	1.540	1.540
Rmerge	0.062	0.195
Number of reflections	20035
<I/σ(I)>	52.2	11.2
Completeness [%]	99.8	99.7
Redundancy	7.5	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	100mM Bis-tris propane pH 7.0, 40% PEG MME 2000, 3% (w/v) xylitol, VAPOR DIFFUSION, HANGING DROP, temperature 294K