3Q41
Crystal structure of the GluN1 N-terminal domain (NTD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-04-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 164.679, 164.679, 147.258 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.240 - 3.400 |
| R-factor | 0.23185 |
| Rwork | 0.229 |
| R-free | 0.27929 |
| Structure solution method | SAD,SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 142.600 | 3.460 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.150 | 0.630 |
| Number of reflections | 32023 | |
| <I/σ(I)> | 12.9 | 2.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | protein in (20mM Tris-HCl pH 7.5, 150mM NaCl) was mixed 1:1 with 4M Formate and 20mM TCEP, temperature 277K, VAPOR DIFFUSION, HANGING DROP |
