3Q34
The crystal structure of YceI-like family protein from Pseudomonas syringae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.647, 103.357, 63.193 |
| Unit cell angles | 90.00, 109.04, 90.00 |
Refinement procedure
| Resolution | 47.481 - 1.700 |
| R-factor | 0.1722 |
| Rwork | 0.170 |
| R-free | 0.20670 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.697 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.700 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.075 | 0.617 |
| Number of reflections | 72488 | |
| <I/σ(I)> | 17.8 | 2.9 |
| Completeness [%] | 99.6 | 98.2 |
| Redundancy | 7.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES pH7.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
