3Q2U
Structure of Human Glioma Pathogenesis-related Protein 1 Reveals Unique loops and surface motifs.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-12 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.077, 79.532, 38.849 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.790 - 1.850 |
| R-factor | 0.13662 |
| Rwork | 0.134 |
| R-free | 0.18421 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q2r |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.888 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.040 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 23229 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 0.085M MES, 25.5% w/v PEG 5000 MME, 0.17M Ammonium sulfate 15% v/v glycerol, pH 6.5, VAPOR DIFFUSION, temperature 293K |
