3Q17
Structure of a slow CLC Cl-/H+ antiporter from a cyanobacterium in Bromide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-30 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 207.209, 207.209, 105.981 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 68.479 - 3.600 |
R-factor | 0.2819 |
Rwork | 0.281 |
R-free | 0.31020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nd0 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.708 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 68.479 | 74.085 | 3.790 |
High resolution limit [Å] | 3.600 | 11.380 | 3.600 |
Rmerge | 0.016 | 0.016 | |
Number of reflections | 29893 | ||
<I/σ(I)> | 8.7 | 3 | 0.5 |
Completeness [%] | 97.8 | 99.7 | 96.9 |
Redundancy | 16.8 | 19.6 | 16.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 291 | 30% PEG400, 0.1 M Tris-HBr, 0.2 M CaCl2 with 14 mM C-Hega-11, 6.2% PEG400 added to protein, pH 8.0, VAPOR DIFFUSION, temperature 291K |