3Q0X

N-terminal coiled-coil dimer domain of C. reinhardtii SAS-6 homolog Bld12p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-10
Detector	MAR225
Wavelength(s)	1.00150
Spacegroup name	P 21 21 21
Unit cell lengths	72.520, 79.800, 89.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.290 - 3.020
R-factor	0.1972
Rwork	0.196
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.290		3.100
High resolution limit [Å]	3.020	10.000	3.000
Rmerge	0.173	0.077	0.742
Number of reflections	10395	337	619
<I/σ(I)>	10.79	20.2	2.6
Completeness [%]	95.8	99.7	62.4
Redundancy	11.23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	298	100 mM TrisHCl, pH 8.5, 200 mM MgCl2, 20% PEG8000, 2% benzamidine, vapor diffusion, temperature 298K, VAPOR DIFFUSION