3Q0K
Crystal structure of Human PACSIN 2 F-BAR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.562, 353.588, 86.045 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 29.630 - 2.600 |
| R-factor | 0.206 |
| Rwork | 0.201 |
| R-free | 0.29180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3haj |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.601 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 44004 | |
| Completeness [%] | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288 | 100mM MgCl2, 100mM NaCacodylate pH 6.5, 18% PEG 3350 (w/v), VAPOR DIFFUSION, SITTING DROP, temperature 288K |
